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Spectrometer scanning n-hexane

2017-03-10View Original

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Scan n-hexane with a spectrometer in the wavelength range of 240–400 nm to determine at which wavelength its absorbance is highest. What reagent should be chosen as a reference? Should I use chromatographically pure n-hexane, or no reference? Do you all have any good methods?
Reply #22017-03-10
We are using the Shimadzu UV-2450
Reply #32017-03-10
This post was last edited by qugd on 2017-3-12 at 22:28. I think there is no need to add a reference liquid, but a blank cuvette should be used as a reference.

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