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Chromatographic peak broadening

2018-11-03View Original

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There are two reasons for peak broadening at the injection port: one is temporal broadening; The second is spatial expansion. The temporal broadening of the peak is determined by the migration speed of the sample vapor from the injection port to the chromatography column; the faster the speed, the narrower the initial peak width. Spatial peak broadening occurs when the sample enters the head of the chromatography column; for example, in the cases of splitless injection and injection on a cold column, the sample partially or completely condenses upon entering the column head. The condensed liquid sample then moves under the action of the carrier gas, spreading over a certain distance – this distance constitutes the initial peak width. If the compatibility between the sample and the stationary phase is poor, droplets may form as well, further increasing the initial peak width; in severe cases, this can lead to the formation of split peaks. I would like to ask the experts: is it true that the narrower the bandwidth, the better?
Reply #22018-11-05
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