How do I perform manual integration for gas chromatography?
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As the title suggests, I’m new to gas chromatography. How do I perform manual integration? How do you tell where the starting point and the ending point are? Thank you
It depends on what model of device you have used; after that, it’s easy to search for ones that allow easy point accumulation.
This post was last edited by qugd on 2019-3-11 21:49. Manual point accumulation is easy. 1. Based on the recorded chromatogram, determine the starting and ending points for integration using the integration rules; once these points are identified, the subsequent steps can be carried out ； 2. If it is grid-lined paper, the small cells can be counted, and the numbers obtained from these counts can be used as integral values ； This is a bit like calculating the points in Go ； 3. Or use graphical methods to measure peak height, half-peak width, and calculate the peak area ； For high peaks in a capillary column, use the peak height data directly ； 4. Alternatively, use scissors to carefully cut out the chromatogram; weigh the cut-out pieces corresponding to the peaks of different components using an analytical balance (the most accurate method). Are these methods sufficient? If you really don’t know how, spend some money to hire me to give you some guidance; the fee won’t be too high. To determine the start and end points for integration, one can refer to basic textbooks on chromatography, or the relevant descriptions in the manuals of chromatography integrators produced by Anja or Shimada.