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(1) Carefully examine the markings on the spherical part of the cylinder’s shoulder before use. Pay special attention to the “next pressure testing time”. And during use, the gas cylinders shall be subject to regular technical inspections as required. Cylinders that have passed their inspection deadline must not be used. (2) When in use, an external inspection should be carried out first, with a focus on the bottle valve, pipe threads, pressure reducer, etc. If leaks, slipping buckles, faulty movement of the hands, or other similar issues are detected, they should be repaired promptly; do not attempt to handle them casually. It is prohibited to tighten the valve stem under pressure or to adjust the shims. Soapy water should be used to check for leaks; open flames must not be used. When a gas cylinder is used together with welding, an insulating material should be placed under the cylinder base to prevent it from becoming charged. Pipes and equipment in contact with gas cylinders must be equipped with grounding devices to prevent combustion or explosion caused by static electricity. When using batteries in winter, frost may form on the valve or pressure regulator; it should be thawed using hot water or steam. It is strictly forbidden to use fire to heat them or to strike the valve with metal objects, nor should the adjustment screws of the pressure regulator be turned forcefully, to prevent large amounts of gas from escaping and causing accidents. (3) During the use and storage of gas cylinders, severe vibrations and impacts should be avoided. When moving oxygen cylinders, it is necessary to handle them gently; specialized lifting frames or carts must be used, and it is prohibited to use steel cables or similar methods to lift them. When in use or storage, the gas cylinder should be secured with railings or supports to prevent it from toppling over. (4) Oxygen cylinders should be kept away from high temperatures, open flames, and molten metal splashes [at a distance of more than 10 meters]. When used in summer, it must not be exposed to direct sunlight. (5) When opening the bottle valve or pressure regulator, do so slowly to prevent explosions or fires in the oxygen cylinder and pressure regulator caused by static electric discharge in high-speed gas streams, heat generated from collisions of solid particles and frictional heating, as well as heat released when the gas is compressed suddenly (adiabatic compression). (6) The oxygen in the oxygen cylinder should not be used up completely; a residual pressure of 0.2–0.3 Mpa should remain to keep the cylinder under positive pressure, and the valve should be tightened to prevent leaks. The purpose is to prevent flammable gases from flowing back into the bottle, and to facilitate the analysis of the gas composition inside the bottle during inflation. (7) The valve of the oxygen cylinder must not be contaminated with grease; tools, gloves, or work clothes that are greasy must not be used to touch the valve or regulator.