Operating procedures for several analytical instruments

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HP-5890 Gas Chromatography Operating Procedures: Startup Steps: Before starting the equipment, first open the carrier gas cylinder, and use the total carrier gas switch on the chromatograph to adjust the carrier gas flow rate to the desired value. Turn on the regulated power supply and wait for the voltage to stabilize at 220V. Turn on the power switch of the host; use the host’s control panel to set the column temperature, injection port temperature, and detector temperature, and input the appropriate signal to the detector being used. After pressing the “DET” key, press the “ON” key; at this point the detector begins to operate, and the magnitude of its base current can be observed through the signal l window. Open the chromatography workstation, use “EDIT” to modify the analysis method, and then use “SAVE” to save it. Set the instrument parameters in “Sample information” and press “RUN” to start the analysis. When the base flow stabilizes at a certain value, the red \"NOT Ready\" light on the window goes out, allowing measurements to be taken. After injecting the sample, press the “START” button on the panel immediately to begin the measurement. At this time, the chromatography workstation measures in synchronization with the host computer. After the measurement, numerical processing is carried out using a workstation, and a report is printed. Shutdown steps: Turn off the switches for \"column temperature,\" \"injection port,\" and \"detector.\" Turn off the “DET” switch. After cooling all the heated parts to room temperature, turn off the carrier gas. Operating procedures for the AA-6501F atomic absorption spectrophotometer – Flame method. Turn on the air compressor switch and set its pressure to 0.4 MPa. Open the valve of the ethylene gas cylinder and adjust its partial pressure to 0.09 MPa. Turn on the main unit switch (if an auto-sampler is used, turn on its power switch as well); after the instrument performs its self-check, press the F1 key to bring the screen to the main menu. Select 1 to enter the flame analysis system. Select 2, set the instrument parameters, then press the F1 key to exit and go to the flame analysis system. Select 3 and set the analysis parameters. Then press F1 to exit. Select 5 to determine the calibration curve, and then analyze the samples. After the sample analysis is complete, turn off the acetylene gas and the air compressor; discharge any remaining acetylene before turning off the main unit. In the Ishiguro furnace method, before starting up, turn on the cooling water and argon, and set the partial pressure of argon to 0.35 MPa. Turn on the power switch of the host, let the instrument perform a self-check, and then press the F1 key after the self-check is complete. Take the screen to the main menu. Select 3 to enter the Ishiguro furnace analysis system. Select 2, set the instrument parameters, then press the F1 key to exit and go to the Ishiguro furnace analysis system. Select 3, set the analysis parameters, then press F1 to exit and enter the Ishiguro furnace analysis system. Select 6 to determine the calibration curve, and then analyze the sample. After the sample analysis is complete, turn off the cooling water and argon, then shut down the main unit. Operating procedures for the PHS-3 pH meter: Immerse the glass electrode in pure water for more than 24 hours before use, clip the electrode to the holder, insert the electrode plug, and tighten the small screw. Check whether the interior of the electrode is filled with a saturated potassium chloride solution, whether potassium chloride crystals have precipitated, and whether there are any bubbles. Keep the capillary at the lower end of the electrode unobstructed; before use, remove the rubber stopper at the upper end and the rubber cap at the lower end of the electrode, clamp the calomel electrode in the clip, and connect the leads to the terminals. Adjust the temperature knob to point at the measured temperature value. Press the mv key. Adjust the positioning screw to ±0.0mv. Turn on the power switch, press the PH button; the indicator light in the upper left corner will light up. Preheat for at least 15 minutes. Place neutral phosphate buffer in the beaker, determine the pH value at that temperature, press the reading switch, adjust the positioning regulator so that the display shows the pH value of the buffer, release the reading switch – the positioning regulator should remain in place. Before testing the sample, calibrate the instrument using a standard buffer solution with a pH value close to that of the water sample, following step \"6\“. Rinse the electrode twice with pure water and then dry it with filter paper. After rinsing with the water sample 6–8 more times, insert it into the water sample, continuously rotate the beaker containing the water sample for one minute, and press the reading switch. Until the pH value of the water sample is read from the display screen. Release the read switch. Turn off the power of the instrument. Operating Procedures for UV-2201 UV-Vis Spectrophotometer: Connect the instrument to a stable 220V power supply, turn on the power and display switches, and wait for the instrument to perform its self-check. Follow the instructions on the display screen to set the instrument parameters and required operating conditions according to the analysis method. Preheat the instrument for 15-30 minutes. After preheating, perform zero extinction reference calibration according to the instructions on the display screen. Pour the colorimetric solution into the cuvette, place it in the colorimeter, cover the sample cell, and press the measurement button to conduct the measurement. After the measurement is complete, press the output button to print the measurement data. Operating Procedures for WFZ800-D2 UV-Vis Spectrophotometer: Before turning on the instrument, first set the \"Range\" knob to the \"0-10%T\" or \"0-100%T\" position, and adjust the \"Concentration\" and \"100%T\" knobs to zero. Connect the instrument to a stable 220V power supply and turn on the power switch. Adjust the wavelength knob so that the counter indicates the selected wavelength value. If the operating wavelength is in the range of 200–400 nm, turn on the \"deuterium lamp\"" ； Within the 400-800nm range, turn on the \"tungsten lamp\". Turn on \"High Voltage\", set the \"Range\" knob to 0-1A or 0-2A, close the sample cell cover, and adjust the \"100%T\" knob so that 1.000 or 2.000 appears on the display. Preheat the instrument for 15-30 minutes. After preheating, pour the colorimetric solution into the cuvette, place it in the colorimeter, and cover it with the sample chamber lid. Push the reference sample into the optical path, then adjust the \"100%T\" knob so that 1.000 or 2.000 appears on the display panel. Push the sample to be tested into the optical path, and record the absorbance value of the sample displayed by the instrument. Operating Procedures for SP-2308 Gas Chromatograph – Startup Steps: Open the main valve of the carrier gas cylinder being used, adjust the output pressure of the pressure regulator to the desired value, then turn on the gas supply switch on the main unit’s panel. Adjust the pressure gauge and flow meter on the main unit to achieve the desired flow rate of the carrier gas. Turn on the main power supply. Turn on the main switch on the temperature control unit. First, set the temperature control values for that unit’s gas II to the desired levels. Then press the gas I heating button on the unit; at this point, both the detector and the vaporization chamber are heated (since the two chambers form one unit). Once they reach the desired temperature, press the column heating button on the unit until the target temperature is achieved. Turn on the power switches for the amplifier and the data processing microcomputer. Follow the menu instructions on the computer screen to carry out the analysis operations. When blowing the base flow, use the \"Analysis Condition Selection\" option on the menu along with the base flow compensation knob on the amplifier to monitor the value of the base flow; once it reaches the desired value, operations and analyses can be performed using the data processing computer workstation. Shutting down steps: Press “End Return” on the menu, then turn off the computer’s power switch. Press the column heating button on the temperature control unit to lower the column temperature to room temperature. Turn off the amplifier power switch. Press the Steam I heating button on the temperature control unit, and turn off the main power supply. Wait until the temperature of the detector drops below 100°C before turning off the carrier gas. Operating Procedures for the 7230G Spectrophotometer: Before turning it on, check that the power socket is connected as specified. When using the instrument, strong light exposure should be avoided. Connect the instrument to a stable 220V power supply, turn on the power switch, and the instrument’s display will show “F7320”. Press the “CLEAR” key, and the instrument displays “YER” to enter year setting. For year setting, use the number keys to enter the corresponding year, then press the “MODE” key; once entered successfully, the instrument will display “MON” to proceed to month setting. For month setting, use the number keys to enter the corresponding month, then press the “MODE” key; once entered successfully, the instrument will display “DA” and proceed to date setting. For date setting, use the number keys to enter the corresponding date, then press the “MODE” key; once entered successfully, the instrument will display “HOU” and proceed to hour setting. For setting the hours, press the number keys to enter the corresponding hour value, then press the “MODE” key; once entered successfully, the instrument will display “MIN” to indicate that it’s time to set the minutes. For minute setting, press the number keys to enter the corresponding minutes, then press the “MODE” key; once entered successfully, the instrument enters time display mode. If it is not necessary to set the year, month, day, hour, or minute, press the “0%?” key after 3.3; the instrument will display “00-00”. Then press the “MODE” key, and the instrument will show the ?(T) status. Adjust the wavelength knob to set the wavelength to the desired value, and let the instrument warm up for 20 minutes. Pour the colorimetric solution into the cuvette, place it in the colorimeter, and cover it with the sample cell lid. Push the reference sample into the optical path and press the “MODE” button to make the instrument display the ?(T) status or A status. Press the “100%?” button until the instrument displays “T100.0” or “A0.000”. Open the sample cell cover and press the “0%?” button until the instrument displays “T0.0” or “A E1”. Close the sample cell cover and press the “100%?” button until the instrument displays “T100.0” or “A0.000”. Push the sample to be tested into the optical path, and the ?(T) value or A value of the sample will be displayed. To record the data of the sample to be tested, simply press the “PRINT” button.