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Centered on the techniques for the separation and purification of biological macromolecules, this book provides a systematic introduction to the basic principles, separation operations, process theory, and applications of the separation and purification of biological products. The content of this book is divided into 12 chapters, namely Introduction, Cell Separation and Lysis, Primary Separation, Membrane Separation, Extraction, Adsorption Separation Techniques and Theories, Liquid Chromatography, Affinity Chromatography, Electrophoresis and Electrochromatography, Protein Refolding, Crystallization and Drying, etc. Chapters 2 and 3 mainly introduce the pretreatment of biological separation processes, as well as primary separation techniques such as precipitation grading and foam separation ; Chapter 4 introduces various membrane separation methods, their characteristics, and their applications in biological separation ; Chapter 5 introduces various extraction methods, particularly biphasic extraction and reverse micelle extraction techniques that can be used for the separation and purification of biological macromolecules ; Chapters 6 to 10 discuss the basic principles, characteristics, fundamental theories, and applications of the core technologies in biological separation processes such as adsorption (including ion exchange), chromatography, electrophoresis, electrochromatography, and protein refolding. They also cover the latest research advancements in this field, constituting the core part of this book ; The last two chapters introduce the basic theories of crystallization and drying and their applications in biological separation. The book is in DJVU format and requires a DJVU browser to download; a small and practical browser is uploaded below. The download address for this book is: http://free.ys168.com/?gtj731003-4 The DJVU version has also been uploaded to this address! It has been uploaded together with the browser to the public 163 mailbox or to the public email address: emuchgtj@163.com. Password: 123456
Does anyone have the third edition of the textbook from 2013?