Bubble problem in gradient elution of macroporous adsorption resin with alcohol solution
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Gradient elution of macroporous adsorption resin with alcohol solution produces bubbles. The higher the alcohol concentration, the more bubbles there are, and the column bed volume is also increasing. I read some posts saying that this phenomenon seems inevitable and can be ignored, but I am still worried about the elution efficiency. In addition, I eluted in a closed column tube of medium pressure chromatography (tube diameter 1.8cm, height 30cm). It is slightly better to switch to an open system, but there will still be bubbles. I wonder if you experts have any good ideas? Please enlighten me! Many thanks!
The bubbles produced by the macroporous adsorption resin should have nothing to do with the alcohol solution, let alone the alcohol concentration! The reason may be that if you drain the resin, bubbles will appear. It is recommended to refill the resin if there are bubbles. Be sure not to drain the resin during use!