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Generally speaking, the smooth progress of laboratory research requires the assistance of various scientific instruments, such as incubators. A microbial incubator is a device used for cell/tissue/in vitro culture. It simulates the growth environment of microorganisms within a living organism or under specific conditions, such as certain temperature, humidity, lighting or other conditions, and CO2 levels. In recent years, with the development of science and technology, CO2 incubators have brought about new changes in the fields of life sciences and biochemical medicine. The incubator is suitable for the cultivation of microorganisms in departments such as environmental protection, health and epidemic prevention, drug inspection, and universities. In terms of chemical properties, carbon dioxide is highly chemically inert and thermally stable (at 20,000°C, only 1.8% of it decomposes); it is non-flammable and generally cannot be ignited. It is an acidic oxide, possessing the general properties of acidic oxides. It is the anhydride of carbonic acid, as it reacts with water to produce carbonic acid. Carbon dioxide is not only a metabolic product of cells but also an essential component for cell growth, and it is related to maintaining the basic pH value of the culture medium. During cell culture, as the amount of CO2 gas released increases, the culture medium becomes acidic; therefore, NaHCO3 is frequently added to adjust the pH value. NaHCO3 has a tendency to release CO2, and the addition of CO2 can inhibit the progress of the reaction. The CO2 concentration in the incubator must be in equilibrium with the NaHCO3 concentration. In cell culture, the proportion of carbon dioxide gas should be 5%; therefore, carbon dioxide gas sensors are used to maintain this balance. Carbon dioxide sensors are commonly used to detect the CO2 concentration inside a chamber, and to transmit the detection results to control devices such as control circuits and solenoid valves. If a low CO2 concentration is detected inside the chamber, the solenoid valve opens, allowing CO2 to enter the chamber. Once the CO2 concentration reaches the set level, the solenoid valve closes, and the CO2 concentration stabilizes. The carbon dioxide gas sensor used in microbial incubators should exhibit good responsiveness, accuracy, and stability. Gongcainet recommends these two models: the new ultra-fast carbon dioxide sensor from the UK-based company GSS – the SprintIR6S. It is designed specifically for applications that require rapid detection of changes in carbon dioxide concentration; its response time is unmatched by any other NDIR CO2 sensor, and it is suitable for measuring CO2 concentrations ranging from 0 to 100%. Models such as the high-range CO2 sensors COZIR-W/ExplorIR-W are available; temperature and humidity outputs can also be optionally added. The measurement ranges are 0-5%, 0-20%, 0-60%, and 0-100%. The refresh rate is slightly low, but it is still suitable for use in incubators; it can also be applied in industries such as diving, industrial safety, and automotive manufacturing. Finally, Gongcaiwang reminds everyone that when using a microbial incubator, it is important to keep both the inside and outside of the incubator clean, and to wash it thoroughly after each use. If not used for an extended period, the inner lining of the chamber walls and the surface of the equipment should be wiped regularly to keep them clean and enhance glass transparency. Do not wipe the outer surface with acids, alkalis, or other corrosive solutions.