Common Questions and Answers about Chromatography
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What is the main cause of non-linear splitting in capillary chromatography with split injection?
Nonlinear shunting? Could you describe in detail that generally, in high-end instruments, the ratio of diversion can be adjusted arbitrarily?
1. The injector temperature is too low, resulting in incomplete vaporization of the sample and fractional splitting. 2. If the injector temperature is too high, certain components may undergo thermal decomposition; some samples may experience catalytic decomposition, or the samples may be partially adsorbed on the inner surface of the injector. 3. The sample was not well mixed or insufficiently mixed before the split point. 4. There is air leakage at the system’s sampling pad, column connector, and other similar locations.