How to age the gas chromatograph column

First, you need to know what column you are using so that you can determine your aging temperature. Usually the aging temperature is 20 to 30 degrees below the maximum allowable temperature of the column. Secondly, the column and detector must be disconnected, otherwise, all the stolen goods during aging will enter the detector, and the detector will be contaminated, which is no fun. ； Thirdly, for detectors with fuel, the fuel gas must be disconnected, but the carrier gas must not be stopped, otherwise the loss will be greater. ； Fourth, for those with rubber diaphragm valves and general metal diaphragm valves, the valve needs to be removed. If it cannot be moved, the column needs to be removed and activated. ； Fifth, the temperature rise should be gentle during aging, otherwise air blockage will easily occur. If the column pressure is too high, column loss will easily occur. ； ~~~~~~ Because the situation is unclear, I will just say this casually.

1. Special post for exchange and discussion of gas chromatography application experience http://bbs.hcbbs.com/thread-383775-1-1.html 2. Installation of capillary tube for gas chromatography http://bbs.hcbbs.com/thread-298882-1-1.html 3. Use and maintenance of chromatographic columns http://bbs.hcbbs.com/thread-309699-1-1.html 4. Introduction to chromatographic analysis by Academician Lu Peizhang http://bbs.hcbbs.com/thread-317429-1-1.html 5. Capillary Chromatography Lecture http://bbs.hcbbs.com/thread-385262-1-1.html 6. Installation methods and steps for gas chromatography columns http://bbs.hcbbs.com/thread-383162-1-1.html 7. Operation skills of gas chromatograph http://bbs.hcbbs.com/thread-381932-1-1.html 8. Common problems and solutions in capillary analysis http://bbs.hcbbs.com/thread-381921-1-2.html 9. Diagnosis and troubleshooting of gas chromatography instrument faults.rar http://bbs.hcbbs.com/thread-342160-1-2.html The above are all information from the information sharing area of ​​the forum analysis version. I will not list them one by one. You can look for them yourself.

I agree with the point of view on the second floor. The explanation is very detailed and the summary is to understand the purpose of aging, the protection of the pillars, and the safety of the laboratory.

Just to add: You need to check how many columns there are in your instrument and then determine the aging temperature. Because each column has a different extreme temperature, do not damage other columns because of one of them.

1. Purpose: It is to completely remove residual solvents and certain volatile substances in the filling. ； On the other hand, it promotes the fixative to be evenly and firmly distributed on the surface of the support. 2. Method: Columns used at room temperature can be directly installed on the chromatograph, connected to the carrier gas, and flushed until the baseline is stable before use. ； If the column is used under high-temperature operating conditions, pass the carrier gas at a temperature slightly higher than the operating temperature for several hours to dozens of hours to completely flush out the volatile substances in the stationary solution. It can be put into use after the baseline is stable.

It depends on what stationary phase is in your column. Different columns have different aging conditions. Generally, the entrance to the detector is opened, and inert gas such as nitrogen is used for aging for about 12 to 24 hours. The aging temperature is 20-40 degrees higher than the operating temperature.

Generally, a new chromatographic column must be aged before use. To age a chromatographic column, you must first know the maximum temperature of the solid phase of the column. The aging temperature should not exceed the maximum temperature. The aging temperature should be about 50 degrees higher than the operating temperature. During the aging process, the port where the column enters the detector must be opened to avoid contaminating the detection room. It is best to pass inert gas for the aging gas, and nitrogen is a good gas source. It should be noted that the chromatographic column should be protected from moisture during use to extend its service life. Furthermore, aging should not be done too frequently, and aging operations should be performed only when the chromatogram is not standardized.

The aging time should be more than 10 hours. When performing column aging, other columns in the chromatography chamber need to be removed.

Not necessarily, it is better to use carrier gas for aging. Including hydrogen carrier gas.

My understanding is that this view is too dogmatic. The aging temperature is 50 degrees higher than the use temperature. If it is a silica gel column, follow this procedure. After two aging cycles, the column will be scrapped. ； If the molecular sieve is used as a permanent gas, its aging is equivalent to no aging if the temperature is higher than the use temperature. At this time, the use temperature is lower, and the aging temperature is 200 degrees...300 degrees higher than the use temperature. Too careful! Personal opinion.

Be careful. Each of our pillars costs more than 3,000 yuan. It’s expensive:lol :lol

Awesome, very well taught, learn from* Got it

It should be enough to put a beard on the column.

The engineer must have received relevant training when the instrument was installed. Generally, the carrier gas should be turned off and the detector end should be about 10 degrees below the maximum operating temperature. The program temperature should be raised and kept lowering after repeated several times, preferably for more than 10 hours!