Regarding the issue of component peaks in chromatography
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When analyzing a certain sample, five peaks, namely A, B, C, D, and E, are obtained; among them, B is the component of interest, while D and E are present in very small amounts, practically zero. For the component being tested around 01, AC is not the component I’m interested in; I also don’t know what it is. Its area is small, so when calculating the content of the sample, AC is not taken into account. Component B can reach 99%. 8 or above. May I ask everyone, is this correct? No, how should it be calculated? Thank you! This post was last edited by Canghai Yili Sha on 2009-2-13 22:19.]
It is recommended that the original poster download some materials on gas chromatography to take a look; there are too many words to count in one sentence
The component resolution is not calculated in that way. That’s all for now.
To calculate the sample content, all components must be taken into account
If only Component B is needed and B is a known sample, then use the external standard method
1. It is recommended that you run a control test with reagent only, to confirm that the issue is caused by the sample itself and not by the solvent. 2. Analyze the two scenarios: one with and one without including those two components, in order to assess the errors in the statistics. 3. Use the same method to analyze your data over a period of time, identify any patterns in the appearance of impurities, and once confirmed, report this to the process engineer so that they can investigate further. You’ve made good progress recently; feel free to visit often
I don’t quite understand it. Is the poster testing the content? Is a liquid phase being used? If so, after confirming that the baseline is stable with no fluctuations, it is best to use the external standard method for quantification; if no standards are available, it is advisable to prepare homemade controls with high purity. If the baseline is unstable, the internal standard method should be used. The reason is that different substances have different response factors at the same detection wavelength, and using peak area normalization alone for quantification will result in significant errors. If no standard is truly available, the normalization method should be used; all peaks other than the solvent peak should be included in the calculations.
Thank you to all the kind friends above! We use gas chromatography with area normalization for calibration.