What causes the high peak height of the solvent in the liquid phase detection?
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When we performed liquid chromatography analysis on solid samples, we found that the solvent peak was much higher than before; this situation persisted even after changing the mobile phase. What could be the reason for this?
1. The composition of the sample changes. 2. The use of a different D lamp results in changes in energy. 3. The amount of sample introduced changes. 4. Impurities cause interference. All of these can lead to changes in the peaks
Thanks to the person above; the first three can be ruled out! ! !
Take a look to see if the retention time of the solvent peak shifts earlier; if it does, the peak height will increase； Furthermore, while keeping the chromatographic conditions unchanged, it is possible for the retention times of various components to advance over time as usage continues ； In addition, the temperature of the analysis room also has a significant impact; generally, as the temperature in the analysis room increases, the retention time decreases.
1. Check according to the ideas of those above to see if there are any related items. 2. If not, check whether your flow rate has changed; you only mentioned that it changes at high levels, but has the peak area changed significantly? 3. It would be best to provide the model of your instrument, the name of the sample being tested, and the concentration of the sample, so that everyone can analyze it together