Liquid phase sample peak problem! ! !
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During liquid chromatography detection, peaks are sometimes flat-topped. So what is the difference between flat-topped peaks and non-flat-topped peaks? Let’s discuss it! ! !
You can't quantify flat-top peaks, right? I don't think so! To put it bluntly, uneven hair is controllable, but flat hair is uncontrollable. Xiao Xiao said that the sharp peaks conform to the rules of chromatography and can be accurately quantified, and the latter can be calculated as an estimated value. If you really want this value, it's better to use another measurement method. Personal opinion.
Our liquid phase does not use a workstation, but uses a processor. The peak produced is a flat peak, and this result is used as the detection result.
1. The flat-top peak in the test seems to be related to the scale of the vertical axis. For specific circumstances, the poster can name the concentration of your sample. 2. Under the same conditions, it depends on which quantitative method you use, area normalization or internal standard, etc. In this way, based on the specific situation, you need to check whether the concentration of your sample is within the quantitative range. 3. Does the poster say that sometimes the flat-top peak refers to the same concentration of the same sample? I suggest that the poster can describe the problem more clearly. At the same time, there is a lot of information about liquid chromatography in this version. It is recommended that you download it and take a look.
During method design, we do not allow flat-head peaks to appear, except for solvent peaks. Flat-head peaks are not accurate enough when calculated by computer software. If flat-head peaks occur, we mainly use split flow to process the sample, and of course we can also reduce the injection volume.