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Spectrophotometer Multiple Choice Questions (Multiple Choice Questions): 1. The UV-visible spectrophotometer is used to characterize the substance based on the response of the measured substance molecules to monochromatic radiation in the UV-visible band range ( ). A. Scattering B. Absorption C. Reflection D. Stimulated radiation 2. After a molecule absorbs external radiation, its energy change is the sum of the following energies (). A. Translation energy B. Vibration energy C. Rotation energy D. Electronic motion energy 3. What are the typical optical components in the ultraviolet-visible spectrophotometry optical path (). A. Convex lens B. Concave mirror C. Prism D. Grating E. Concave lens F. Polarizer G. Filter 4. Internationally, UV-visible spectrophotometers are generally divided into () according to their instrument structure () A. Single beam B. Quasi-double beam C. Double beam D. Dual wavelength 5. What are the photoelectric conversion devices commonly used in spectrophotometers () A. LED B. PDA C. CCD D. CMOS E. PMT F. Photocell G. Phototube H. Photosensitive transistor I. Photoresistor 6. What indicators are mainly referred to in the pairing of cuvettes () A. Height B. Shape C. Parallelism of the light-transmitting surface D. Optical path E. Spectral characteristics F. Temperature characteristics 7. What can be used for wavelength verification of UV-visible spectrophotometer () A. NaI B. Praseodymium and rubidium filter C. Copper sulfate solution D. Holmium glass (or solution) E. Potassium dichromate solution F. Deuterium lamp G. Mercury lamp 8. What are the quantitative methods of UV-visible spectrophotometer () A. Absolute method B. Standard method C. Specific absorption coefficient method D. Standard curve method E. Regression equation method F. Simultaneous equation method G. Differential spectrophotometry 9. How to prevent photolysis of the test sample () A. Put in a glass bottle B. Put in a brown bottle C. Wrap in black paper D. Put in a plastic bottle 10. The spectral bandwidth of the UV-visible spectrophotometer used for drug testing is required to be less than () A. 1nm B. 2nm C. 3nm D. 4nm True or False question: 1. Substances with similar absorption spectra have similar structures. 2. The molecular absorption spectrum of a substance is a linear spectrum. 3. Noise limit is the lower limit of the sample analysis concentration of the UV-visible spectrophotometer instrument. 4. Tungsten lamp is the light source used in the ultraviolet region of UV-visible spectrophotometer. 5. For the pairing of cuvettes, single-beam spectrophotometers have lower requirements than double-beam spectrophotometers. 6. During analysis and testing, the solution injected into the cuvette must be level with the mouth of the cuvette to completely absorb the incident light. 7. When the spectrophotometer is preheating, the sample chamber cover must not be opened to prevent strong light in the room from irradiating the photodetector. 8. When measuring absorbance, the sample does not need to be too concentrated. 9. The test of protein and sugar content is completed in the visible light area. 10. Certain substances can be qualitatively analyzed using UV-visible spectrophotometer. This post was last edited by caozhikui on 2009-3-6 20:52 ]