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Basic working principle of UV spectrophotometer: The basic working principle of the UV spectrophotometer is similar to that of the infrared spectrometer. It uses UV-visible light of a certain frequency to illuminate the organic matter being analyzed, causing the transition of valence electrons in the molecule, which will be selectively absorbed. A set of spectra whose absorption changes with wavelength, reflecting the characteristics of the sample. In the range of ultraviolet and visible light, for a specific wavelength, the degree of absorption is proportional to the concentration of the component in the sample, so the measured spectrum can be used for qualitative analysis, and based on the comparison of the absorption with a standard sample of known concentration, quantitative analysis can also be performed. Characteristics and main uses of UV spectrophotometer: The wavelength range of UV-visible spectrometer is 0.2-0.8 microns (corresponding to wave number 50000-12500 cm-1), which is widely used in organic chemistry research. It is usually used for substance identification, purity inspection, and research on organic molecular structure. In terms of quantification, it can determine the content of each component in compounds and mixtures with relatively complex structures. It can also determine the dissociation constant of substances, the stability constant of complexes, indicate the end point in molecular weight identification and microtitration of substances, and be used as a detector in high-performance liquid chromatography.