How to prepare this indicator?
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I will need the dichlorofluorescein indicator for an open-ended experiment next time, but after searching through many sources I still don’t know how to prepare it. If any expert knows how, please give me some guidance.
This reagent is available for purchase... Here is the manufacturer’s website: http://www.aladdin-reagent.com/thir.asp?Rea_SubID=16134
Fluorescein indicator: Take 0.1 g of fluorescein and dissolve it in 100 ml of ethanol to obtain the solution. http://bbs.cqvip.com/html/358/130021.shtml describes the preparation of 50 indicators; please refer to it. This post was last edited by zhangyong6404 on 2009-3-21 at 14:28.]
Product number: 13060; Alternative names: 4ˊ,5ˊ-Dichlorofluorescein; English name: 4ˊ,5ˊ-Dichlorofluorescein; CAS#: 2320-96-9; Molecular formula: C20H10Cl2O5; Molecular weight: 401.20; Structure: Orange powder. It is orange in color and exhibits yellow-green fluorescence when dissolved in ethanol and dilute alkaline solutions; it is slightly soluble in glycerin and diols, almost insoluble in oils, fats, and waxes, and insoluble in water and dilute acids. Its strongly alkaline solution turns purple when heated. Its sodium salt is C20H8Cl2Na2O5, C.I.45365. It is used as an adsorption indicator in the silver quantitation method for the determination of halogens. This product should be stored sealed and protected from light. Provide the concentration, and you can prepare it yourself
Well, I’ve taken a look; they are all common methods for preparing indicators. Thank you so much!