What are the methods for determining 3-nitro-4-hydroxybenzoic acid?
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Does anyone here know the method for determining 3-nitro-4-hydroxybenzoic acid? What are they? Please share if you can; thank you!
Name: 3-Nitro-4-hydroxybenzoic acid Purity: 99% Packaging: 25kg/barrel Structural formula: CAS: 616-82-0 Molecular formula: C7H5NO5 Molecular weight: 183.12 Chinese name: 3-Nitro-4-hydroxybenzoic acid; 4-Hydroxy-3-nitrobenzoic acid English name: 4-hydroxy-3-nitrobenzoic acid; 3-nitro-4-hydroxybenzoic acid Product quality Appearance: Light yellow crystalline powder Visual inspection Melting point: 182.0–184.0°C General method for determination of freezing point Purity: ≥98.0% (HPLC), Liquid chromatography Moisture content: ≤0.5%, General method for determination of moisture in organic substances
I would like to ask about the specific methods and testing conditions for determining content using liquid chromatography. Our company has also purchased this raw material recently and needs to have it tested; we haven’t yet figured out the appropriate testing method.
1. Based on the properties of this substance, a C-8 column will suffice. If you have a liquid chromatograph, it is recommended that you select an appropriate column and then gradually reduce the proportion of organic phase from 90% down to 10% until the main peaks become separated. 2. Contact the manufacturer to obtain information from the raw material suppliers. 3. Look into the production process to identify the main raw materials, so as to check for any residues of those materials. I’m only giving general suggestions here; you’ll need to carry out the actual work yourself. It shouldn’t be too difficult