Determination of mPEG molecular weight using GPC method

2009-04-01View Original

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Use the GPC method to measure the molecular weight of mPEG (polyethylene glycol monomethyl ether) (between 5000 and 20000). The column I use is three chromatographic columns, but the mobile phase has not been selected well because the main peak is always followed by a small peak connected to the main peak, so the measured molecular weight is definitely inaccurate. Now I want to ask what mobile phase should be chosen to separate the main peak from other impurities so that the molecular weight can be measured accurately? Or what kind of column and mobile phase should be chosen~~~~ Please give an expert answer~~~~ Thank you~~~~~

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Determination of mPEG molecular weight using GPC method

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