correction factor
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The gas chromatography workstation used by our company employs the area normalization method for quantification. A standard sample containing different components was prepared to determine the correction factors for each component; once the sample was injected, the correction factors appeared directly in the workstation, and we used these factors right away. I wonder whether this measured correction factor is an absolute or a relative correction factor? If it is the relative correction factor fi/s, how is this factor determined? If the measured value is the absolute correction factor fs, why use the absolute value rather than the relative one? Thank you!
If an internal standard is added to the standard sample you prepare – a substance that is not present in the sample being analyzed but is added artificially and can be completely separated from other components – then the absolute correction factor for the internal standard is set to 1. The absolute correction factors of the other substances in the sample are compared with this value, and the computer calculates the relative correction factors using the formula Fis = mi/Ai/ms/As. The absolute correction factor, on the other hand, is simpler: Fi = mi/Ai. I think that if there are multiple components in the sample being analyzed, it’s possible to use one of these components as a reference, assuming that its absolute correction factor is 1. By comparing the correction factors of the other components with this value, their respective relative correction factors can be determined, which simplifies the calculations. Last edited by whcj668 on 2009-4-7 17:22
It is not the internal standard method. It is the area normalization method.
Actually, the correction factor can be calculated by oneself: it is obtained by dividing the concentration of the standard sample by its peak area (which is the absolute correction factor), and that’s exactly how we calculate it!