In liquid chromatography, how can substances with similar polarities be separated effectively?
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In liquid chromatography analysis, how can the following benzoic acid derivatives be separated? 4-Degraded benzoic acid, 3-nitro-4-hydroxybenzoic acid, 3-amino-4-hydroxybenzoic acid – This can also be extended to the question of how, in liquid chromatography, substances with similar polarities can be effectively separated I hope some experienced experts can give me some advice~~~
I haven’t done this analysis. What you mean is that the separation effect is good, in other words, the retention times of the various peaks differ from each other; in that case, you just need to look at the factors that affect retention times. Flow rate, temperature, and the composition of the mobile phase can all influence retention times – give it a try on your own
“We’ve tried various conditions such as flow rate, temperature, and the composition of the mobile phase. For the mobile phase, we currently only have methanol and acetonitrile available; since there are no more polar mobile phases available, no matter how these conditions are changed, it’s not possible to separate these substances. It’s frustrating! I wonder if there are other ways we can approach this?
It’s not easy to separate this using the liquid phase; it should be separated and purified through a specific process before analysis. 3-Amino-4-hydroxybenzoic acid can be easily purified