Dangers of using non-chromatographically pure reagents as mobile phase in chromatography
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What are the hazards of using non-chromatographically pure reagents as the mobile phase in chromatography? In case of an emergency, is it acceptable to use analytically pure reagents as the mobile phase for analysis occasionally?
Anyway, you need to perform suction filtration and ultrasonication before using the mobile phase; so if the analytical requirements aren’t very high, you can go ahead and do it
To improve the separation efficiency, **furan was used as the mobile phase with AR today. Previously, suction filtration and ultrasound were used, along with methanol as the mobile phase, for injecting samples in order to detect background absorption. However, when **furan** was used as the mobile phase, the column pressure rose extremely high, almost reaching its limit; as a result, it was necessary to switch back to methanol in order to reduce the pressure on the column. The reason for such a sharp change in column pressure is not yet clear~~~
It is not allowed to use AR as a substitute for chromatographically pure material; chromatographically pure material goes through strict treatment processes. Generally, using AR leads to increased pressure, as it contains many impurities that can easily clog the chromatography column. Last edited by chengjingbao on 2009-4-10 12:50.]
Previously, when reading materials on the differences between analytically pure and chromatographically pure solvents, it was mentioned only that the impurities present in analytically pure solvents might affect the detection sensitivity under chromatographic testing conditions; however, the overall purity of analytically pure solvents is still comparable to that of chromatographically pure solvents. So what exactly are the differences between analytically pure and chromatographically pure solvents? I hope everyone will continue to discuss it~~~
In principle, it is not allowed! If it’s really urgent, use analytically pure material; the analysis data obtained in this case can only be used as reference data!
As for the difference: it lies not in purity or concentration, but in cleanliness. That is, in terms of impurity content and type.