The issue of retention value
Thread Content
May I ask an expert: In TLC analysis, why is the Rf value equal to 0? What’s the reason? Urgent
Rf value: In thin-layer chromatography, it is the ratio of the distance from the origin to the center of a spot to the distance from the origin to the front edge of the solvent front. Also known as Rf, it is a parameter used in chromatography to indicate the position of movement of a component. It is defined as the ratio of the solute migration distance to the mobile phase migration distance. Under certain chromatographic conditions, the Rf value of a specific compound is constant; therefore, it is possible to identify a compound based on its Rf value. Use the previous sample to conduct a comparative analysis, and then check whether there are any changes before and after the analysis, including the reagents used and so on