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[Share] Lecture slides on molecular luminescence analysis (comparison of fluorescence and phosphorescence)

2009-04-14View Original

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Luminescence Analysis Method Lecture Notes (Comparison of Fluorescence and Phosphorescence) (No duplicates found after search) Luminescence Analysis Method (Comparison of Fluorescence and Phosphorescence Analysis – Basic Concepts) (For beginners only) A brief overview of the lecture content: 1. Mechanisms of fluorescence and phosphorescence 2. Excitation spectrum and fluorescence (phosphorescence) spectrum 1. Fluorescence (Phosphorescence): Photoemission – How should the wavelength of the incident light be chosen? 3. The relationship between excitation spectra and emission spectra III. The relationship between fluorescence generation and molecular structure 1. Conditions necessary for a molecule to produce fluorescence (1) Having an appropriate structure ; (2) It has a certain fluorescence quantum yield. Fluorescent quantum yield = number of photons emitted / number of photons absorbed. 2. Structure of compounds and fluorescence. IV. Factors affecting fluorescence intensity. V. Instrument structure. VI. Fluorescence analysis methods and applications. VII. Applications of phosphorescence analysis. Click to download

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