What effect does column temperature have on separation?
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This post was last edited by ask123456 on 2010-3-11 14:55 What impact does column temperature have on separation? What should you pay attention to when choosing column temperature?
1# Beauty 1 Hello, buddy, you don’t seem to have a good understanding of the basic knowledge of chromatography. I suggest you read books on chromatography and learn it carefully.* one time. You can also download some information from this forum. Very helpful to you!
The separation effect becomes worse when the column temperature is higher, and better when the column temperature is lower. Determination of column temperature: On the premise that all samples to be analyzed are separated, select the column temperature as low as possible. This can protect the chromatographic column. Generally, the maximum column temperature is close to the temperature of the component with the highest boiling point in the sample to be measured.
This post was last edited by ask123456 on 2010-3-12 10:16 2# zhijielin Thanks, brother, I really don’t understand, I’m still learning* 3# wolf1147 Very useful, thanks