Measure molecular weight using GPC
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Why is there a strong peak after the solvent peak when measuring molecular weight using GPC?
There might be bubbles in the mobile phase. The mobile phase must be thoroughly degassed!
Thanks, I’m new to this and don’t quite understand it.
I have another question: what causes the two or three inverted peaks that appear after the solvent peak? In the measured GPC spectrum, the horizontal axis represents the elution time, while the vertical axis represents Mv. What does Mv mean? It seems a bit contradictory, because molecules with a higher molecular weight flow out first, so the corresponding time value on the horizontal axis should be smaller. But then why is the corresponding value of Mv on the vertical axis also smaller?
It is recommended that the original poster find a book to read first, such as: Gel Chromatography and Its Applications (available online).