Fine chemicals analysis and testing

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This post was last edited by huawei on 2010-6-3 15:19 In chromatography, the stationary phase (solid or liquid) filled in a glass tube or stainless steel tube is called the stationary phase； A phase that moves from top to bottom (usually gas or liquid) is called the mobile phase ； The tube (glass tube or stainless steel tube) containing the stationary phase is called a chromatographic column. When the sample mixture in the mobile phase passes through the stationary phase, it will interact with the stationary phase. Due to the differences in properties and structures of each component, the type and strength of the interaction with the stationary phase are also different. Therefore, under the action of the same driving force, different components have different retention times in the stationary phase, and thus flow out of the stationary phase in different orders. In chromatographic analysis, when the mobile phase carries the sample through the stationary phase of the chromatograph, Interactions between sample molecules and stationary phase molecules occur, causing the sample to be partitioned between the mobile phase and the stationary phase. Components with greater interaction force with stationary phase molecules move forward slower, and components with smaller interaction force with stationary phase molecules move forward faster. After a certain distance, due to repeated distribution, components with small differences in original properties (boiling point, polarity, etc.) can also be well separated.