Regarding manual integration of chromatography
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Everyone: What principles should be followed when manually integrating chromatograms? For example, how are the starting point and ending point determined?
It’s best to understand it thoroughly on your own; for manual integration, it is recommended to consult the relevant chapter in the classic book \"Practical Gas Chromatography Analysis\" by Jian Yixing: http://bbs.hcbbs.com/viewthread.php?tid=363831&highlight=%CA%B5%D3%C3%C6%F8%CF%E0%C9%AB%C6%D7
In the early days, chromatographs would produce graphs that were recorded by data loggers; there were no integrators, and chromatographic peaks had to be manually integrated by drawing them as triangles using a pen and ruler. All you need to look at is the height and width of the peak!
Analyze each specific problem individually; it’s best to start by using a standard sample so that you can have a clear idea of what to expect
The principle of manual integration is from the baseline to the corner of the peak
It is best to use a standard sample and perform manual integration tests on it; by doing this several times, the necessary practical knowledge can be acquired.