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C13 NMR spectroscopy analysis: Where does the C13 in this sample come from?

2021-07-30View Original

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Dear Haihao friends, I have a question from a layperson’s perspective: For samples used to generate C13 MRI spectra, where does the C13 in these samples come from? Isn’t it all C12?
Reply #22021-08-01
This post was last edited by qugd on 2021-8-1 at 18:45. C13 is a stable isotope of carbon; it coexists with C12 in carbon-containing compounds in nature, accounting for about 1%. Carbon-14 is the radioactive isotope; it also exists in nature, but due to decay, its amount gradually decreases in dead plants. The C13 NMR spectrum is a good method for studying the structure of organic compounds. Read more on the spectroscopy aspects of organic chemistry, and you’ll understand what’s going on.
Reply #32021-08-02
Well, your answer is extremely comprehensive – you even mentioned C14. That means nothing needs to be added; a direct measurement is sufficient! :lol
Reply #42021-08-02
That’s right; there are specialized theories and methods for analyzing the C13 spectrum in nuclear magnetic resonance spectra, as is known to those familiar with this field.

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