The pipette’s principle of \"more pressure, less tapping\"”
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I saw some experience shared by a colleague, which stated that when using a micropipette, one should apply \"firm pressure with light taps\" to achieve more accurate liquid addition. I don’t quite understand this statement; could any teacher explain it to me?
Is there any teacher who can help answer this?
It may refer to the process of drawing up viscous liquids, where pressure is applied to the second stop point of the pipette when drawing up the liquid, and then the pressure is released to allow the liquid to flow into the tip of the pipette. When dispensing the liquid, press to the first stop point of the pipette (do not press all the way down), wait for a moment, then release it; there is no need to worry about the liquid remaining in the tip at this point. When draining, pressing to the first stop point corresponds to the set volume you have collected. You can look up on the Internet the correct way to use a pipette; there are detailed videos available.
What he means by “heavy pressure, light strikes” might refer to “second-gear suction.” One row” ； For drawing in conventional liquids, it is generally done using \"suction on level 1, discharge on level 2\"”~