Seeking help from experts regarding the determination of the conversion rate of water-soluble solvents using liquid chromatography

Sure. If liquid chromatography can only detect the conversion rate of organic solvents, and your reaction solvent is water, then you can centrifuge the product, dry it into a solid, dissolve it in an appropriate organic solvent, and then use liquid chromatography for analysis. This allows you to measure the conversion rate of the product. Please be careful to choose an appropriate organic solvent, and maintain the stability of the product during centrifugation and dissolution. .

If you are to centrifuge the product, dry it into a solid, and dissolve it in an organic solvent for analysis by liquid chromatography, then you need to ensure that the organic solvent you choose is suitable for your product and enables good separation and detection under liquid chromatography conditions. Additionally, if you wish to compare the conversion rates under different reaction conditions, you need to maintain the same concentration of the product solution in each experiment. This can be achieved by preparing solutions of the same concentration based on the molar amount of the product when dissolving the solid product. However, please note that before performing liquid chromatography analysis after converting the product into a solid and dissolving it to the same concentration, it is necessary to first confirm whether the stability of the product has been affected by the solidification and dissolution processes. Some compounds may undergo degradation or other reactions during curing or dissolution, leading to distorted liquid chromatography results. Therefore, in practical applications, it is essential to conduct a series of control experiments and carefully verify the accuracy and reliability of the results. .

Okay, thanks boss. I’m going to give it a try in practice