Lactose-specific column

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Chengdu Moore Scientific Instruments Co., Ltd. specializes in providing various specialized chromatography columns for analysis and testing. Such as **special columns, motherwort-specific columns, honey-specific columns, heparin sodium-specific columns, lactose-specific columns, and so on** ； We offer a full range of specifications and models, and can also customize specialized chromatography columns according to customer requirements. Next, I will introduce the lactose-specific column. If it needs to be purchased. Welcome to search for Chengdu Moore Scientific Instruments Co., Ltd. The Lac-Amino column specifically designed for lactose is obtained by bonding amino groups to a silica gel column using high-purity bonding reagents. During the separation under the chromatographic conditions specified in the pharmacopoeia, the detection of lactose and sucrose met the pharmacopoeial requirements for resolution; the number of theoretical plates calculated based on the lactose peak was always above 8000, which is far higher than the requirements set by the pharmacopoeia. Chromatography column: Lac-Amino, 5 μm, 120 Å, 4.6×300 mm. Mobile phase: ACN:H2O = 70:30 (v/v). Flow rate: 1.0 mL/min. Column temperature: 45°C. Detection: Differential (40 °C). Sample: System suitability solution (1.0 mg/mL lactose + 1.0 mg/mL sucrose). Injection volume: 10 μL. Features of the lactose-specific column: 1. The packing material of the Lac-Amino column consists of uniform spherical particles, which ensures maximum coverage of functional groups in a single layer and thus guarantees good peak shapes. 2. By strictly controlling the surface chemical modification conditions, reliable reproducibility between columns is ensured. 3. The bed density of the Lac-Amino column, achieved through its unique loading technique, is uniform and stable, thereby ensuring the highest column efficiency. 4. It meets the requirements of pharmacopoeias regarding the matrix of chromatography columns, and fully satisfies the separation requirements specified by pharmacopoeias for sample analysis. Ordering information for lactose-specific columns: Instructions for using lactose-specific columns, column specifications. The Seikon lactose-specific Lac-Amino bonded-phase column is based on silica gel of high purity and good mechanical stability; amino groups are bonded to the silica gel using high-purity bonding reagents, resulting in a carbon content of 4.0%. The filler consists of uniform spherical particles with a pore size of 120 Å and a specific surface area of 300 m2/g. This bonding phase features a unique innovative design that enables maximum coverage of functional groups on a single layer. By strictly controlling the synthetic reaction conditions, reliable reproducibility between columns can be ensured. The Lac-Amino column beds prepared using the unique homogenization loading technique exhibit uniform and stable density, thereby ensuring the highest column efficiency. The Lac-Amino column is an amino-bonded silica gel chromatography column, highly recommended for the detection of the excipient lactose in Part IV of the 2015 edition of the Chinese Pharmacopoeia. Figure 1 shows the chromatogram obtained by testing lactose and sucrose standard solutions using a Lac-Amino 4.6×300 mm chromatography column, under the chromatographic conditions specified in Section IV – Determination of Lactose Content of the 2015 edition of the Chinese Pharmacopoeia. Safety precautions: Lactose-specific columns are usually operated under high pressure. If the pipeline connections are not tight, it will lead to leaks of organic solvents and the samples being injected, thereby affecting the health of the operators. In the event of a leak, appropriate gloves should be worn to handle it. Additionally, when opening the chromatography column, appropriate protective measures should be taken to prevent tiny silica gel particles from entering the respiratory tract. Figure 1: Chromatogram of sucrose and lactose reference standards. Installation and operation of the chromatography column: During transportation or when not in use, both ends of the chromatography column are always sealed with plugs. When connecting the chromatography column to the chromatography instrument system, first remove the plugs at both ends. Please make sure that the flow direction of the mobile phase is consistent with the direction indicated on the column. Unless there are special reasons, such as the need to reverse the chromatography column for flushing in order to remove dirt blocked at the inlet end of the column, it is recommended that users always follow the direction indicated on the column when connecting it. Since the connection of the chromatography column is part of the entire chromatography process, excessive tightness of the sealing collar, improper installation, or a mismatch between the sealing collar and the column port can all lead to solvent leakage. Please connect the chromatography column to the sealing sleeve following the steps below, in order to integrate the chromatography column into the HPLC system: (a) For pipelines used for the first time, sequentially fit the pipeline fittings and sealing sleeves onto the pipeline with an outer diameter of 1/16”. The wide end of the sealing collar should face the pipeline joint. (b) Insert the tubing firmly into the interface of the chromatography column, slide the sealing collar and the tubing connector forward so that the threads of the tubing connector match those of the column port, and then tighten the tubing connector. If the pipeline is made of polymer material, proceed to step (d) ； If it is a metal tube or wire, please proceed to (c). (c) After forcing the pipeline into the column end fitting, use a 1/4” wrench to tighten the nut further. (d) Apply the above method to the other end of the chromatography column. The new Lac-Amino chromatography column is stored in pure acetonitrile. During storage and transportation, the silica gel filler may dry out; in such cases, it is recommended to wash the chromatography column at a low flow rate using pure acetonitrile in an amount of 5 to 10 times the column volume. Next, the column can be equilibrated with the aqueous solution of 70% acetonitrile, which is the mobile phase required for lactose testing in Part IV of the 2015 edition of the Chinese Pharmacopoeia, until the baseline stabilizes. If the column pressure and baseline fluctuations are large, it may be due to bubbles entering the chromatography column. At this time, the chromatography column can be flushed at a higher flow rate (1.0 mL/min) for 2–5 minutes. Samples and mobile phase: To prevent clogging of the chromatography column, all samples and solvents must be filtered through a 0.45 μm or 0.22 μm filter membrane before use. The lactose-specific column is designed specifically for the detection of lactose, a excipient, as specified in Part IV of the 2015 edition of the Chinese Pharmacopoeia, and testing is carried out using the mobile phase system prescribed by the pharmacopoeia. The mobile phase needs to be degassed before use; a simple method for degassing is to ultrasonicate the mobile phase under vacuum created by a water pump for 5 minutes. Care of the chromatography column: Avoid using the column at pH levels below 2.0 or above 8.5. A high pH will dissolve the silica gel, causing some or all of the bonded phase to detach from the surface of the silica gel, which leads to a decrease in separation efficiency and changes in retention times. To achieve the best separation results and extend the column’s lifespan, please use a mobile phase with a pH within the range of 2.0 to 7.5. Pressure: Although lactose-specific columns can be used at pressures up to 4000 psi, the normal operating pressure should be below 2500 psi. Operating under high pressure for a long time can damage the chromatography column and the infusion pump. Since pressure arises from flow velocity, the maximum flow velocity will be limited by the pressure that the system can withstand. Generally, column pressure gradually increases as the usage time of the chromatography column increases. A sudden increase in pressure indicates that the sieve plate at the inlet end of the chromatography column is blocked. In this case, it is recommended to reverse the connection of the chromatography column and rinse it with an appropriate solvent. Temperature: The maximum operating temperature is 60°C. Operating at high temperatures (>75 °C) for extended periods can damage the chromatography column, a problem that is particularly pronounced under high pH conditions (>8.5). Column cleaning: After multiple uses, impurities from certain samples may adsorb onto the inlet sieve or packing. When it accumulates to a certain extent, pressure increases along with a broadening of the peak shape. In such cases, the chromatography column can be activated and rinsed using pure acetonitrile, the recommended storage solvent, at a low flow rate. Storage: When not in use for an extended period, please rinse the chromatography column with pure acetonitrile in a volume at least 20–30 times that of the column volume, and then store it. Each chromatography column is equipped with two removable plugs during transportation. To prevent the column bed from drying out, please seal both ends of the chromatography column with plugs, and store it at room temperature.