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1. Under certain conditions, the retention times of the two components are 12.2 s and 12.8 s respectively; calculate the resolution. The column length required to achieve complete separation, i.e., R=1.5. (Known n=3600) 2. Determination of the sensitivity of the thermal conductivity cell detector: 1 mL of pure benzene is injected; the height of the benzene chromatographic peak is 4 mV, and the half-width at half-maximum is 1 minute. The flow rate of the gas at the column outlet is 20 mL/min. Determine the sensitivity of this detector (the density of benzene is 0.88 g/mL). If the instrument noise is 0.02 mV, calculate its detection limit. 3. The retention times of n-heptane and n-hexane on a certain chromatography column are 94 s and 85 s, respectively; the retention time of air on this column is 10 s. The number of theoretical plates obtained is 3900. What is the resolution of these two compounds on this column? 4. During the gas analysis of a certain tank, the areas of the various peaks were: methane 32,884, nitrogen 4,290, oxygen 584. Determine the air response area: nitrogen 64,823, oxygen 14,970; calculate the nitrogen and oxygen contents. Is there an accurate calculation?
This post was last edited by qugd on 2015-6-18 21:30. Is this an exam question? Why not calculate it yourself using the information you’ve learned? This is a quite standard calculation in instrumental analysis; are you planning to copy it from the internet?
The answer is that I don’t know if it’s correct, so I want to verify it. .