【Daily Question】Chemical Engineering Principles 375: Reference Solution (May 13)
Thread Content
This post was last edited by Zaihui Kangqiao on 2016-6-13 at 16:59. The Chemical Engineering Theory section is launching a \"One Question per Day\" activity starting today, aimed at helping everyone reinforce their basic knowledge in chemical engineering. Subsequent series will include those on \"Principles of Chemical Engineering\", \"Mass Transfer and Separation\", \"Thermodynamics in Chemical Engineering\", and \"Chemical Process Engineering\". We hope you will give it your active support! Wishing everyone a happy Christmas! Answers to the questions in the \"One Question per Day\" activity can be viewed directly; the thread will be closed after 1 day ! To encourage everyone’s continued participation this year! Participation earns 3 wealth points, with an additional 4 wealth points for correct answers~~~ Short answer question: What is the purpose of using a reference solution in photometric measurements? Eliminate the influence of non-measuring substances such as solvents and reagents on the absorption of incident light
The effect of a substance on the absorption of incident light
In spectrophotometry, the function of the blank (reference) solution is to adjust the zero point of absorbance (A) and to counteract the effects of the cuvette, solvent, etc., on the incident light.
The effect of a substance on the absorption of incident light
Generally, the solvent used in testing the sample is employed as the reference solution, with the aim of deducting the absorption values caused by the solvent and thereby eliminating the background effect
1. Eliminate the impact of the absorption cell walls on light. 2. Improve accuracy
Eliminate the influence of non-measuring substances such as solvents and reagents on the absorption of incident light
Adjust the zero point of the instrument, that is, set its absorbance to zero
Zero point used to adjust the instrument’s transmittance: victory:
What is the role of a reference solution in photometric measurements? Answer: To eliminate the effect of non-measuring substances such as solvents and reagents on the absorption of incident light.