The commonly used inoculation device previously relied on flame inoculation, and improvements are now being sought
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It seems that the sterility level of this vaccination method isn’t very high; I’d like to improve it. Could experts recommend some solutions?
A sterile inoculation loop or a microbial inoculation gun can be considered; these devices help to increase the sterility of the inoculation process. At the same time, operations should also be carried out under a laminar flow hood to ensure an aseptic working environment. .
Can this large-scale production be used? I’ve done some research, but I don’t quite understand it. Could you provide a more detailed explanation?
The four-valve assembly + peristaltic pump approach can be considered, which is suitable for inoculating strains of 500 ml or more into the primary seed tank.