【3D Process Flow】Summary Post--Two-Phase Extraction

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【3D Process Flow】Summary Post – Useful for learning about the process flow: https://bbs.hcbbs.com/thread-5711616-1-1.html ---------------------------------------------------------- Biphasic extraction: Biphasic extraction (ATPE) is a mild liquid-phase extraction technique used in the pharmaceutical industry for the separation and purification of biomacromolecules such as proteins, enzymes, and nucleic acids. The core principle involves using two hydrophilic polymers (such as polyethylene glycol/dextran) or polymers/salts (such as PEG/phosphates) to form a two-phase system that is immiscible in aqueous solution; the target product is selectively distributed between the two phases based on differences in their surface properties. The process flow mainly includes: preparation of the biphasic system → mixing and extraction of the feed solution → phase separation → recovery of the target phase → post-treatment of the product. This technology has advantages such as mild operating conditions (room temperature and normal pressure), good biocompatibility, and ease of scale-up, making it particularly suitable for the extraction of heat-sensitive bioactive substances.