Vacuum blood collection tubes, serum separation gel, anticoagulants, SST tubes, PRP beauty tubes, PCT tubes
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Around the 1920s, Americans invented vacuum blood collection tubes to replace syringes for drawing blood. The American company BD acquired this patent and brought it to commercial use. Around the 1930s, it began to be commercialized and made available for use by the U.S. Navy during World War II. Since then, various technologies such as test tube cleaning techniques, silicidation techniques, butyl rubber stopper technologies, two-way blood collection needles, butterfly-shaped blood collection needles, PET plastic tubes, and separation gels have been invented, continuously improving the processes and technologies related to blood collection tubes. By the 1970s and 1980s, vacuum blood collection tube technology had become widely used in developed Western countries. It was an inevitable trend for vacuum tubes to replace syringes in blood collection, as well as for the standardization and normalization of blood specimen processing. Blood collection tube giants such as BD in the United States, Grenna (Phicotec) in Austria, and Terumo in Japan lead the industry’s development and hold a large share of the global market. The development of blood collection tube products in our country began in the 1990s, marked by the **Science and Technology Commission establishing a research team for the industrialization of blood collection tube production techniques at Wuhan University and setting up a university-run factory. Currently, in the domestic market, separation gel is mainly used in SST tubes and regular serum tubes found in vacuum blood collection tubes. In fact, PRP treatment tubes produced by companies such as BD in the United States and Ficote in Austria, as well as CPT tubes, hold new market prospects. In different blood collection tubes, one of the most important additives is serum separator gel. 【Product Name】 Generic name: Serum Separator Gel (radiation-resistant) 【Specifications】 20KG/barrel, 25KG/barrel 【Product Properties】 This product appears as a colorless, transparent gel with a faint odor and contains no impurities ； It is physiologically inert, insoluble in water, with a relative density of 1.045–1.065 g/cm³ and a viscosity of 100,000–300,000 centipoise. Its volatiles content is ≤1.5%. It exhibits excellent thixotropic and insulating properties. For other properties, please refer to the product test report. 【Scope of Application】This product is specifically designed for manufacturing disposable vacuum blood collection tubes and syringe-type blood collection tubes, and serves as an essential material for the preservation, transfer, and transportation of high-quality serum and blood samples in clinical testing. 【Precautions】 Our separating gel is a patented product with unique ingredients; please do not mix it with products from other suppliers to avoid defective products and unnecessary losses for you ； The recommended operating temperature range for the separation gel is -20 to +90°C. The viscosity of this product changes significantly with variations in ambient temperature. An excessively low ambient temperature may make it difficult to apply the adhesive. It is recommended that when the ambient temperature is below 15°C, the material be heated in a water bath to 60–70°C to reduce viscosity and improve the efficiency of adding glue ； If “stringing” occurs during glue application at room temperature, the glue application temperature can be increased slightly, or the strands can be broken off by vibration ； This product has been treated under high vacuum, so there is virtually no air trapped in the colloid. Stirring is generally not recommended during transfer or addition of the adhesive, as stirring can cause a large amount of air to get trapped in the colloid ； After being added to a test tube, this product was subjected to an inverted test at 60°C under vacuum for more than 20 consecutive days, with the flow distance of the colloid plane remaining ≤5 mm. It can be guaranteed that when transported in containers at 60°C for 20 days consecutive, there will be no flow exceeding 5 mm, which will not affect the normal use of blood collection tubes. Nevertheless, we still recommend that blood collection tubes containing separation gel not be inverted during long-distance transportation ； It is recommended not to touch the adhesive directly with your hands. If it sticks to the skin, first use a clean metal sheet to scrape it off; wipe away most of the adhesive with old newspaper, then clean it using organic solvents such as DMC or D4. Alternatively, you can use cleaning powder mixed with sawdust or dish soap, and rinse it thoroughly with water. Cleaning of the separation gel feed hopper: When switching to separation gel from a different manufacturer, or when a hopper that has not been in use for an extended period and has been exposed to air needs to be put back into use, it must be cleaned first. The cleaning method is as follows: first, use a stainless steel shovel to remove the glue from the surface around the feed hopper, then clean it with a brush dipped in solvents such as DMC and D4, and finally wipe it clean with a cloth dipped in the same solvents. The feeding tube also needs to be cleaned to prevent colloidal contamination. [Warnings and Precautions] 1. Do not use if any impurities or foreign substances are found in the serum separator gel, if there is an abnormal odor or color, or if the expiration date has passed. 2. This product is an inert substance, safe and non-toxic. 3. This product should not be mixed with products from other manufacturers, as mixing may cause the colloid to separate or become cloudy. 4. Exceeding the recommended storage temperature limits may damage the quality of the separation gel. 【Special storage conditions and methods】Unless otherwise indicated on the packaging, it is generally recommended to store in a sealed condition at a temperature of 0–40°C (32–104°F), avoiding direct exposure to air and sunlight. 【Validity Period】36 months
EDTA dipotassium anticoagulant 【Product Name】 Dipotassium ethylenediaminetetraacetate dihydrate
【Packaging Specification】 Plastic bottle, 500 g per bottle
【Product Properties】 White crystalline powder; odorless; readily soluble in water; hygroscopic. Molecular weight: 404.6. pH value: 4.8 ± 1 (5% aqueous solution, g/ml). Solubility in water: ≥60% (g/ml at 25°C). Stable at room temperature. The main content of EDTA dipotassium in our company is ≥99.0%. For other indicators, please refer to the product test report. 【Scope of application】EDTA salts can protect the cellular components of blood, do not affect white blood cell counts or size, have minimal impact on red blood cell morphology, and can inhibit platelet aggregation; they are suitable for routine hematology tests. Apart from being used for the separation and testing of platelets, it is not suitable for coagulation tests or platelet function tests. It is also unsuitable for the determination of calcium ions, potassium ions, sodium ions, iron ions, alkaline phosphatase, creatine kinase, and leucine aminopeptidase, as well as for PCR tests. The International Council for Standardization in Haematology (ICSH) recommends dipotassium EDTA as the preferred anticoagulant for blood cell counting and differentiation. Compared to potassium EDTA, it has lower solubility and a slower anticoagulation rate.
【Packaging Specification】 Plastic bottle, 500 g per bottle
【Product Properties】 White crystalline powder; odorless; readily soluble in water; hygroscopic. Molecular weight: 404.6. pH value: 4.8 ± 1 (5% aqueous solution, g/ml). Solubility in water: ≥60% (g/ml at 25°C). Stable at room temperature. The main content of EDTA dipotassium in our company is ≥99.0%. For other indicators, please refer to the product test report. 【Scope of application】EDTA salts can protect the cellular components of blood, do not affect white blood cell counts or size, have minimal impact on red blood cell morphology, and can inhibit platelet aggregation; they are suitable for routine hematology tests. Apart from being used for the separation and testing of platelets, it is not suitable for coagulation tests or platelet function tests. It is also unsuitable for the determination of calcium ions, potassium ions, sodium ions, iron ions, alkaline phosphatase, creatine kinase, and leucine aminopeptidase, as well as for PCR tests. The International Council for Standardization in Haematology (ICSH) recommends dipotassium EDTA as the preferred anticoagulant for blood cell counting and differentiation. Compared to potassium EDTA, it has lower solubility and a slower anticoagulation rate.
[Precautions] The key to preparing whole blood test specimens is ensuring thorough anticoagulation; therefore, after blood collection, the tube must be inverted 5–8 times as soon as possible to prevent the formation of small blood clots. Especially when the ambient temperature during blood collection is above 25°C, the mixing of blood with dipotassium EDTA must be timely and thorough; otherwise, it can easily lead to blood coagulation or localized clotting. EDTA salt anticoagulation is not an irreversible form of anticoagulation; therefore, tests must be conducted within 24 hours after blood sampling using EDTA anticoagulated tubes, otherwise it may lead to errors in the test results. The storage time for anticoagulated specimens with EDTA salts is as follows: for white blood cell counts, they can be stored at 4°C for 48 hours, and at 23°C for no more than 24 hours. The platelet count can be preserved at 4°C for 24 hours. Anticoagulation requires an adequate amount of EDTA, but excessive EDTA may cause changes in the morphology of blood cells; therefore, it is particularly important to accurately control the amount of dipotassium EDTA added. EDTA salts can inhibit platelet aggregation, but they cannot completely prevent it. In a few patients, the platelet count is below normal levels as shown by blood routine tests; this may be caused by pseudo-aggregation of platelets. In such cases, sodium citrate anticoagulant can be used for platelet testing to verify the result. This situation has nothing to do with the quality of the EDTA anticoagulant; rather, it is closely related to the patient’s blood characteristics and the procedures involved in clinical testing. Potassium EDTA can be used in combination with sodium fluoride and separation gel; when using potassium EDTA together with sodium fluoride, 1.2–2.0 mg of potassium EDTA per milliliter of blood is sufficient, along with 2–4 mg of sodium fluoride. When using EDTA dipotassium in combination with separation gel, the dosage of dipotassium remains unchanged; 0.8–1.2 g of separation gel per test tube is sufficient. When used in conjunction with separation gel, factors such as anticoagulation effect, silicification of the tube walls, centrifugation conditions, and the quality of the separation gel all affect the efficiency of blood separation. It is recommended to use it in combination with the separation gel produced by our company to obtain high-quality plasma samples. Recommendation for irradiation sterilization after adding to blood collection tubes: Use gamma radiation with a dose of 8–25 kGy. The irradiation dose can be determined from the initial colony count. Potassium EDTA blood anticoagulant 【Warnings and Precautions】 It must not be used if potassium EDTA contains impurities, foreign substances, an abnormal odor or color, is severely hygroscopic, or has passed its expiration date. Do not use the potassium EDTA solution if it becomes cloudy. This product is a chemical reagent with strong corrosive properties; it must not be ingested, and direct contact with the hands is prohibited. When it comes into contact with the skin, rinse thoroughly with plenty of water. If it gets into the eyes, rinse them immediately with plenty of clean water; in an emergency, seek medical attention right away for examination and treatment.
Potassium EDTA anticoagulant 【Product Name】Potassium dihydrogen ethylenediaminetetraacetate •2H2O 【Molecular Formula】EDTAK3·2H2O 【Packaging】Plastic bottle, 500g per bottle 【Product Properties】White crystalline powder, odorless, soluble in water, hygroscopic; molecular weight 442.56, pH value 7.3±1 (5% aqueous solution, g/ml); solubility in water ≥60% (g/ml, 25°C). Stable at room temperature. The main content of EDTA tripotassium in our company is ≥99.0%. For other indicators, please refer to the product test report. 【Primary Use】This product is specifically designed for external anticoagulation of blood; it serves as an anticoagulant additive in clinical blood testing, and is used for the pre-treatment of blood samples (anticoagulation) during blood collection and testing procedures. 【Scope of application】EDTA salts can protect the cellular components of blood, do not affect white blood cell counts or size, have minimal impact on red blood cell morphology, and can inhibit platelet aggregation; they are suitable for routine hematology tests. Apart from being used for platelet separation tests, it is not suitable for coagulation tests or platelet function assays, nor for the determination of calcium ions, potassium ions, sodium ions, iron ions, alkaline phosphatase, creatine kinase, and leucine aminopeptidase, or for PCR tests. The International Committee for Standardization in Hematology (ICSH) recommends EDTA tripotassium salt as the anticoagulant of choice for blood cell counting and classification. Compared to disodium EDTA, it has a higher solubility and a faster anticoagulation rate.
Potassium EDTA 【Precautions】 The key to preparing whole blood test samples is thorough and complete anticoagulation; therefore, the sample must be inverted 5–8 times as soon as possible after blood collection to prevent the formation of small blood clots. Especially when the temperature in the blood collection environment is above 25°C, the mixing of blood with potassium EDTA must be thorough and prompt; otherwise, it can lead to blood clotting or localized clotting. EDTA salt anticoagulation is not an irreversible form of anticoagulation; therefore, tests must be conducted within 24 hours after blood sampling using EDTA anticoagulated tubes, otherwise it may lead to errors in the test results. The storage time for anticoagulated specimens with EDTA salts is as follows: for white blood cell counts, they can be stored at 4°C for 48 hours, and at 23°C for no more than 24 hours. The platelet count can be preserved at 4°C for 24 hours. Anticoagulation requires an adequate amount of EDTA, but excessive EDTA may cause changes in the morphology of blood cells; therefore, it is particularly important to accurately control the amount of tripotassium EDTA added. EDTA salts can inhibit platelet aggregation, but they cannot completely prevent it. In a few patients, the platelet count is below normal levels as shown by blood routine tests; this may be caused by pseudo-aggregation of platelets. In such cases, sodium citrate anticoagulant can be used for platelet testing to verify the result. This situation has nothing to do with the quality of the EDTA anticoagulant; rather, it is closely related to the patient’s blood characteristics and the procedures involved in clinical testing. Potassium EDTA can be used in combination with sodium fluoride and separation gel; when using potassium EDTA together with sodium fluoride, 1.2–2.0 mg of potassium EDTA per milliliter of blood is sufficient, along with 2–4 mg of sodium fluoride. When using EDTA tripotassium in combination with separation gel, the dosage of tripotassium remains unchanged; 0.8–1.2 g of separation gel per test tube is sufficient. When used in conjunction with separation gel, factors such as anticoagulation effect, silicification of the tube walls, centrifugation conditions, and the quality of the separation gel all affect the efficiency of blood separation. It is recommended to use it in combination with the separation gel produced by our company to obtain high-quality plasma samples. Recommendation for irradiation sterilization after adding to blood collection tubes: Use gamma radiation with a dose of 8–25 kGy. The irradiation dose can be determined from the initial colony count. 【Warnings and Precautions】 EDTA tripotassium must not be used if it contains impurities, foreign substances, an abnormal odor or color, is highly hygroscopic, or has passed its expiration date. Do not use the EDTA tripotassium solution if it becomes cloudy. This product is a chemical reagent with strong corrosive properties; it must not be ingested, and direct contact with the hands is prohibited. When it comes into contact with the skin, rinse thoroughly with plenty of water. If it gets into the eyes, rinse them immediately with plenty of clean water; in an emergency, seek medical attention right away for examination and treatment. 【Storage Conditions】This product should be stored in a cool, dry place in a sealed container, away from light, and must not become damp. The aqueous solution of this product can be stored sealed at room temperature under aseptic conditions. It is recommended to use the aqueous solution of this product immediately after preparation, and not to store it in solution form for an extended period of time. 【Validity Period】3 years
Heparin Sodium Anticoagulant 【Product Name】Heparin Anticoagulant 【Packaging Specifications】Plastic bottles, 10g per bottle, 20g per bottle, 50g per bottle 【Product Standard】Q/WHDS 003-2016 【Product Properties】White amorphous powder, odorless, soluble in water, hygroscopic; average molecular weight is 15,000; stable at room temperature. The potency of our product is ≥150 IU/mg, with an anhydrous potency of ≥160 IU/mg. Other parameters are controlled in accordance with the standards for the active pharmaceutical ingredient. 【Scope of Application】 1. This product is suitable for the collection and anticoagulation of blood samples used in clinical biochemical tests and emergency biochemical tests, as well as for the collection and anticoagulation of blood samples used in certain hemorheology tests. 2. When determining ion levels in blood through clinical tests, heparin is recommended as an anticoagulant because it is least likely to interfere with the measurement of other ions. ? 3. This product is not a pharmaceutical and cannot be used as an injection; direct injection into humans or animals is prohibited.
Sodium heparin 【Precautions】 To ensure adequate blood anticoagulation, it is necessary to invert the container and mix the contents 5–8 times as soon as possible after blood sampling, especially when the temperature in the sampling environment is above 25°C. Proper and timely mixing of the blood with heparin is essential; otherwise, blood clotting or localized clot formation may occur. Heparin anticoagulation is not an irreversible form of anticoagulation; therefore, tests using blood collected with heparin anticoagulation should be completed within 6 hours, otherwise it may lead to inaccurate test results. Heparin may be involved in the metabolism of cellular enzymes and ions; therefore, the dosage of heparin used may affect the test results. The dose of heparin added should ensure adequate anticoagulation of the entire sample as well as its individual components, thereby allowing most plasma parameters to be measured again within 6 hours, especially sensitive indicators such as AST, ALT, TBIL, DBIL, and GGT. Heparin can be used together with the separation gel; factors such as anticoagulation effect, silicification of the tube walls, centrifugation conditions, and the quality of the separation gel all affect the efficiency of blood separation. It is recommended to use it in combination with the blood separation gel produced by our company to obtain high-quality plasma samples. Recommendation for irradiation sterilization after adding to blood collection tubes: Use gamma radiation with a dose of 8–25 kGy. The irradiation dose can be determined from the initial colony count. 【Warnings and Precautions】 Do not use the product if it contains impurities or foreign objects, has an abnormal odor or color, or has passed its expiration date. If the heparin solution becomes cloudy, it may be contaminated or spoiled; do not use it any longer. This product is a biologic agent that is safe, non-toxic, and harmless. 【Storage Conditions】This product should be stored in a cool, dry place in a sealed container, away from light, and must not become damp. The aqueous solution of this product can be stored sealed at 0-4°C under aseptic conditions, with a maximum storage period of 7 days. It is recommended to use the aqueous solution of this product immediately after preparation, and not to store it in solution form for an extended period of time.
Lithium heparin anticoagulant 【Product Name】Lithium heparin 【Packaging Specifications】Plastic bottles, 10g per bottle, 20g per bottle, 50g per bottle 【Product Properties】White amorphous powder, odorless, soluble in water, hygroscopic; average molecular weight is 15,000; stable at room temperature. The lithium heparin of our company has a potency of ≥150 IU/mg, while its anhydrous form has a potency of ≥160 IU/mg. Other parameters are controlled in accordance with the standards for heparin sodium drug substance. 【Scope of Application】 1. This product is suitable for the collection and anticoagulation of blood samples used in clinical biochemical tests and emergency biochemical tests, as well as for the collection and anticoagulation of blood samples used in certain hemorheology tests. 2. When determining ion levels in blood through clinical testing, lithium heparin is recommended as an anticoagulant because it least likely to interfere with the measurement of other ions. 3. This product is not a pharmaceutical and cannot be used as an injection; direct injection into humans or animals is prohibited.
Precautions for Lithium Heparin Anticoagulant [Warnings and Precautions] Lithium heparin must not be used if it contains impurities or foreign substances, has an abnormal odor or color, or has passed its expiration date. If the lithium heparin solution becomes cloudy, it may be contaminated or spoiled; do not use it any further. This product is a biologic agent that is safe, non-toxic, and harmless. 【Storage Conditions】This product should be stored in a cool, dry place in a sealed container, away from light, and must not become damp. The aqueous solution of this product can be stored aseptically at 0-4°C in a sealed container, with a maximum storage period of 7 days. It is recommended to use the aqueous solution of this product immediately after preparation, and not to store it in solution form for an extended period of time. 【Validity Period】3 years. Desheng is a established blood testing reagent company with 14 years of experience in research and production, and it has in-depth expertise in the study of additives for blood collection tubes.
Sodium citrate anticoagulant 【Product name】Calcium ion-chelating anticoagulant, sodium citrate, citric acid sodium salt 【Packaging size】500g/bottle 【Product properties】White crystalline powder that is prone to absorbing moisture. 【Uses】Can be used in the food and beverage industry as a flavoring agent and stabilizer ； Used in the pharmaceutical industry as an anticoagulant, expectorant, and diuretic ； In the detergent industry, it can replace sodium trimetaphosphate as an additive for non-toxic detergents ； It is an excellent chelating/complexing agent from a chemical perspective, and its industrial applications take advantage of this property. It is also used in brewing, injectable solutions, photographic chemicals, electroplating, etc. 【Validity Period】3 years. Desheng is a established blood testing reagent company with 14 years of experience in research and production, and it has in-depth expertise in the study of additives for blood collection tubes.